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Collagen IV 抗体

Cited in 4+ publications. This 小鼠 单克隆 anti-Collagen IV antibody (Clone LP46) specifically detects Collagen IV in WB, IHC, IHC (fro), IHC (p) 和 ICC. The antibody is reactive with 人, 大鼠, Pig 和 Cow samples.
产品编号 ABIN335405
发货至: 中国
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Quick Overview for Collagen IV 抗体 (ABIN335405)

抗原

See all Collagen IV (COL4) 抗体
Collagen IV (COL4) (Collagen, Type IV (COL4))

适用

  • 96
  • 48
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  • 2
  • 2
人, 大鼠, Pig, Cow

宿主

  • 64
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小鼠

克隆类型

  • 74
  • 30
单克隆

标记

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  • 2
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This Collagen IV antibody is un-conjugated

应用范围

  • 62
  • 48
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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

克隆位点

LP46
  • 特异性

    Human, rat, pig, cow.

    纯化方法

    Purified

    免疫原

    1043 is a mouse monoclonal IgG2b antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta.

    亚型

    IgG1
  • 应用备注

    1043 recognizes the extracellular - basement membranes. 1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration, 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • Schapers, Pauwels, Havenith, Smeets, van den Brandt, Bosman: "Prognostic significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder." in: Cancer, Vol. 66, Issue 12, pp. 2583-8, (1991) (PubMed).

    Cleutjens, Havenith, Kasper, Vallinga, Bosman: "Absence of type IV collagen in the centre of the corneal epithelial basement membrane." in: The Histochemical journal, Vol. 22, Issue 12, pp. 688-94, (1991) (PubMed).

    Cleutjens, Havenith, Beek, Vallinga, Ten Kate, Bosman: "Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines." in: The American journal of pathology, Vol. 136, Issue 5, pp. 1165-72, (1990) (PubMed).

    Visser, van der Beek, Havenith, Cleutjens, Bosman: "Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia." in: Histopathology, Vol. 10, Issue 2, pp. 171-80, (1986) (PubMed).

  • 抗原

    Collagen IV (COL4) (Collagen, Type IV (COL4))

    别名

    Collagen Type IV
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