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FAS 抗体 (Extracellular Domain) (PE)

This anti-FAS antibody is a 小鼠 单克隆 antibody detecting FAS in WB, FACS 和 ELISA. Suitable for Cat.
产品编号 ABIN323507
发货至: 中国

Quick Overview for FAS 抗体 (Extracellular Domain) (PE) (ABIN323507)

抗原

See all FAS 抗体
FAS (TNF Receptor Superfamily, Member 6 (FAS))

适用

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Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This FAS antibody is conjugated to PE

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA
  • 抗原表位

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    Extracellular Domain

    特异性

    Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived recombinant feline rfeFas extracellular domain.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Cat

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
  • 抗原

    FAS (TNF Receptor Superfamily, Member 6 (FAS))

    别名

    CD95 / FAS

    背景

    Name/Gene ID: FAS
    Family: TNF Receptor

    Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95

    基因ID

    355

    UniProt

    P25445

    途径

    p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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