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- 抗原 See all IgG2 products
- IgG2
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- It has been selected for its ability to recognize IgG2 in immunohistochemical staining and Western blotting.
- 纯化方法
- Affinity Chromatography
- 免疫原
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Native Protein IgG2There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, IgG2-B, and IgG2-A/B, which differ by the disulfide connectivity at the hinge region. IgG2, the second largest part of IgG isotypes in humans, comprises 20-25 % of the main subclass and is the prevalent immune response against carbohydrate-/polysaccharid antigens.
The antibody is a mouse monoclonal antibody raised against IgG2. - 亚型
- IgG
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Mouse anti-Human IgG2 (Fc Region) antibody
ELISA Monoclonal HP6002 IgG1 unconjugated
Mouse anti-Human IgG2 (Fd Region) antibodyELISA, WB, IHC, ICC Monoclonal HP6014 IgG1 unconjugated
Mouse anti-Human IgG2 (Fc Region) antibody (PE)ELISA Monoclonal HP6002 IgG1 PE
Mouse anti-Human IgG2 (Fc Region) antibody (Biotin)ELISA Monoclonal 31-7-4 IgG1 Biotin
Mouse anti-Human IgG2 (Fd Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6014 IgG1 Biotin
Mouse anti-Human IgG2 (Fc Region) antibody (HRP)ELISA, WB, IHC Monoclonal 31-7-4 IgG1 HRP
Mouse anti-Human IgG2 (Fd Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6014 IgG1 FITC
Mouse anti-Human IgG2 (Fc Region) antibody (FITC)ELISA, FLISA Monoclonal 31-7-4 IgG1 FITC
Mouse anti-Human IgG2 (Fc Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal 31-7-4 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG2 (Fd Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6014 IgG1 HRP
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- 应用备注
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 500 µg/mL
- 缓冲液
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- 有效期
- 12 months
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- 抗原
- IgG2
- Abstract
- IgG2 产品
- 物质类
- Antibody
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