VGF 抗体 (AA 330-449)
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- 抗原 See all VGF 抗体
- VGF (VGF Nerve Growth Factor Inducible (VGF))
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抗原表位
- AA 330-449
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VGF antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF)
- 序列
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -D LLLQYLLQGG ARQRGLGGRG LQEAAEERES AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ NALLFAEEED GEAGAEDKRS QEETPGHRRK EAEGTEEGGE EEDDEEMDP
- 特异性
- The antibody is a mouse monoclonal antibody raised against VGF. It has been selected for its ability to recognize VGF in immunohistochemical staining and western blotting.
- 交叉反应
- 小鼠, 大鼠
- 纯化方法
- Protein A + Protein G affinity chromatography
- 免疫原
- Recombinant VGF Nerve Growth Factor Inducible (VGF) corresdonding to Asp330~Pro449 with N-terminal His and GST Tag
- 克隆位点
- 9#
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product VGF Primary Antibody
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- 应用备注
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Western blotting: 0.5-2 μg/mL
1:250-1000 Immunohistochemistry: 5-20 μg/mL
1:25-100 Immunocytochemistry: 5-20 μg/mL
1:25-100 Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- VGF (VGF Nerve Growth Factor Inducible (VGF))
- 别名
- VGF Nerve Growth Factor Inducible (VGF 产品)
- 别名
- VGF antibody, Gm1052 antibody, VGF nerve growth factor inducible antibody, VGF antibody, Vgf antibody
- 背景
- NERP1, NERP2, Neuro-Endocrine Specific Protein VGF, Neuroendocrine Regulatory Peptide
- 途径
- Hormone Transport, Carbohydrate Homeostasis
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