CD103 抗体
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- 抗原 See all CD103 (ITGAE) 抗体
- CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
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适用
- 人, 猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD103 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognises the CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of intra-epithelial lymphocytes:- 40 % of lamina propria T-cells, 30 % of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1 % bone marrow cells and less than 0.7 % PBL.
- 纯化方法
- Affinity Chromatography on Protein A.
- 免疫原
- Human Intestinal lymphocyte. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- 克隆位点
- 2G5-1
- 亚型
- IgG2a
- Top Product
- Discover our top product ITGAE Primary Antibody
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anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibody
ITGAE 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal ITGAE-2063 unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibodyITGAE 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal ITGAE-2474 unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibodyITGAE 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibodyITGAE 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal Ber-ACT8 unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibody (APC)ITGAE 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal Ber-ACT8 APC
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) (AA 671-917) antibodyITGAE 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) (AA 19-200) antibodyITGAE 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibodyITGAE 适用: 小鼠 FACS, IHC, IP, IHC (fro) 宿主: 仓鼠 Monoclonal 2E7 unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) (AA 57-171) antibodyITGAE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B8 unconjugated
anti-Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibody (FITC)ITGAE 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal Ber-ACT8 FITC
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- 应用备注
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Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 5 x 10e5 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100). Recommended positive control tissue: intestine.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.1 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
- 别名
- CD103 (ITGAE 产品)
- 别名
- A530055J10 antibody, CD103 antibody, aM290 antibody, alpha-E1 antibody, alpha-M290 antibody, HUMINAE antibody, integrin alpha E, epithelial-associated antibody, integrin subunit alpha E antibody, Itgae antibody, ITGAE antibody
- 背景
- CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20 % of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150 kDa alpha chain and a 120 kDa beta chain. Integrin alpha E is expressed by approximately 1 % of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.Synonyms: HML-1 antigen, ITGAE, Integrin alpha-E, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen
- 基因ID
- 3682
- NCBI登录号
- NP_002199
- UniProt
- P38570
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