(4 references)-
- 抗原 See all IGF1R 抗体
- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
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抗原表位
- Chain alpha
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IGF1R antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 特异性
- This antibody recognises CD221, a 155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane betasubunits. Clone 1H7 recognises an epitope in the alpha subunits of CD221, demonstrated by Western blotting. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221. We recommend the use of BM2226LE for this purpose.
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 克隆位点
- 1H7
- 亚型
- IgG1
- Top Product
- Discover our top product IGF1R Primary Antibody
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anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 251-350) antibody
IGF1R 适用: 人, 小鼠, 大鼠, 犬 FACS 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibodyIGF1R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1165), (pTyr1166) antibodyIGF1R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1161) antibodyIGF1R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 51-77) antibodyIGF1R 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 1335-1366), (C-Term) antibodyIGF1R 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB01458 unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (C-Term) antibodyIGF1R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 660-760) antibodyIGF1R 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr980) antibodyIGF1R 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (Tyr1346) antibodyIGF1R 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot. Immunohistochemistry on paraffin sections: 1/10-1/40, This product requires proteindigestion pre-treatment of paraffin sections. Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Positive Control: Pancreas, placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "IGF1-R inhibition and liposomal doxorubicin: Progress in preclinical evaluation for the treatment of adrenocortical carcinoma." in: Molecular and cellular endocrinology, Vol. 428, pp. 82-8, (2016) (PubMed).
: "The detection of EpCAM(+) and EpCAM(-) circulating tumor cells." in: Scientific reports, Vol. 5, pp. 12270, (2015) (PubMed).
: "Liposomal doxorubicin-based treatment in a preclinical model of adrenocortical carcinoma." in: The Journal of endocrinology, Vol. 213, Issue 2, pp. 155-61, (2012) (PubMed).
: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, (2010) (PubMed).
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: "IGF1-R inhibition and liposomal doxorubicin: Progress in preclinical evaluation for the treatment of adrenocortical carcinoma." in: Molecular and cellular endocrinology, Vol. 428, pp. 82-8, (2016) (PubMed).
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- 抗原
- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
- 别名
- CD221 / IGF1R (IGF1R 产品)
- 背景
- There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor (also known as CD221) prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor
- 基因ID
- 3480
- NCBI登录号
- NP_000866
- UniProt
- P08069
- 途径
- RTK signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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