This antibody detects endogenous levels of total Synaptotagmin I/II protein.
纯化方法
Immunoaffinity Chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Synaptotagmin I/II around the phosphorylation site of Threonine 202/199 (R-K-Tp-L-N).
Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Synaptotagmin 1 is a 60 kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding. Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity. Synaptotagmin 2 belongs to the synaptotagmin family of multi-domained, integral membrane proteins of synaptic vesicles which are thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.Synonyms: SYT1/SYT2, Synaptotagmin I/II