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Phospholipase C gamma 2 抗体 (pTyr1217)

The 兔 多克隆 anti-Phospholipase C gamma 2 antibody is suitable to detect Phospholipase C gamma 2 in samples from 人, 大鼠 和 小鼠. It has been validated for IHC (p).
产品编号 ABIN319283
发货至: 中国
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Quick Overview for Phospholipase C gamma 2 抗体 (pTyr1217) (ABIN319283)

抗原

See all Phospholipase C gamma 2 (PLCG2) 抗体
Phospholipase C gamma 2 (PLCG2)

适用

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人, 大鼠, 小鼠

宿主

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克隆类型

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多克隆

标记

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This Phospholipase C gamma 2 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    pTyr1217

    特异性

    This antibody detects endogenous levels of PLC-gamma 2 only when phosphorylated at Tyrosine 1217.

    纯化方法

    Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    The antiserum was produced against synthesized phosphopeptide derived from Human PLC-gamma 2 around the phosphorylation site of Tyrosine 1217 (F-L-Yp-D-T).
  • 应用备注

    Immunohistochemistry (1/50-1/100).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody (in aliquots) at-20 °C.
  • 抗原

    Phospholipase C gamma 2 (PLCG2)

    别名

    PLCG2

    背景

    Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2

    基因ID

    5336

    NCBI登录号

    NP_002652

    UniProt

    P16885

    途径

    RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling
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