ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-XXX-Val motif. The proteins in this family function as receptors for Wnt and are generally coupled to G proteins. Frizzleds are members of the G protein-coupled receptor superfamily. Frizzled-2 is expressed in the fetal kidney and lung and in the adult ovary and colon. Frizzled-2 mediates the Wnt/cGMP/Ca2+ pathway. It binds Wnt proteins and signals by activating the release of stored calcium. Frizzled-2 expression is regulated by Angiotensin II. Activated frizzled-2 suppresses the activity of protein kinase G, and activates NFAT-dependent transcription, the phosphatidylinositol pathway and calcium sensitive enzymes.Synonyms: Fz-2, FzE2, hFz2