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FZD2 抗体 (Extracellular Domain)

FZD2 适用: 人, 大鼠, 小鼠, Cow, 犬, 马, Hamster, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1048611
发货至: 中国
  • 抗原 See all FZD2 抗体
    FZD2 (Frizzled Family Receptor 2 (FZD2))
    抗原表位
    • 9
    • 8
    • 7
    • 7
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    适用
    • 42
    • 17
    • 14
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠, Cow, 犬, 马, Hamster, Pig
    宿主
    • 40
    • 2
    • 2
    • 1
    克隆类型
    • 42
    • 3
    多克隆
    标记
    • 27
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FZD2 antibody is un-conjugated
    应用范围
    • 33
    • 22
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human Frizzled 2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD7 (67 % ), OR1A2 (56 %), NFATC2 (50 %).
    预测反应
    Percent identity with other species by BLAST analysis: Human, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig (100%) Sheep, Rabbit (94%) Opossum (89%) Turkey, Chicken, Platypus, Lizard, Xenopus (83%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 18 amino acid peptide from 3rd extracellular domain of human Frizzled 2. Percent identity with other species by BLAST analysis: Human, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig (100%), Sheep, Rabbit (94%), Opossum (89%), Turkey, Chicken, Platypus, Lizard, Xenopus (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product FZD2 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 - 10 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    FZD2 (Frizzled Family Receptor 2 (FZD2))
    别名
    FSD2 / Frizzled 2 (FZD2 产品)
    别名
    BG01711 antibody, CG9739 antibody, D-Fz2 antibody, D-fz2 antibody, DFZ2 antibody, DFz-2 antibody, DFz2 antibody, Dfrz2 antibody, Dfz2 antibody, Dfz[[2]] antibody, Dm Fz2 antibody, Dmel\\CG9739 antibody, FZ2 antibody, Fz2 antibody, dFz2 antibody, dfz(2) antibody, dfz2 antibody, dfz2r antibody, frz2 antibody, fz-2 antibody, fz8 antibody, zfz2 antibody, zg08 antibody, cb383 antibody, wu:fb70g09 antibody, FZD2 antibody, fzE2 antibody, hFz2 antibody, Fz-10 antibody, Fzd10 antibody, cFz-2 antibody, frizzled2 antibody, fz2 antibody, xfz2 antibody, AL033370 antibody, AW456835 antibody, Fz10 antibody, Mfz10 antibody, Mfz10a antibody, frizzled 2 antibody, frizzled class receptor 2 antibody, frizzled class receptor 2 S homeolog antibody, fz2 antibody, fzd2 antibody, FZD2 antibody, Fzd2 antibody, fzd2.S antibody
    背景
    Name/Gene ID: FZD2
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2
    基因ID
    2535
    途径
    WNT signaling
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