ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
TIS11B (tetradecanoyl phorbol acetate-inducible sequence 11b), also known as ZFP36L1, BRF1 (butyrate response factor 1), ERF1 (EGF response factor 1), cMG1, Berg36 or RNF162B, is a member of the tristetraprolin family. Tristetraprolin (TTP), or TIS11, is a zinc-binding protein encoded by the immediate-early response gene, Zfp-36. TIS11B, a relative of TTP, localizes to the nucleus and may function as a transcription factor involved in regulating the growth factor response. It is an evolutionarily conserved protein containing two C3H1-type zinc fingers and a repeating Cys-His motif. TIS11B is an mRNA binding protein and is known to interact with the 3'-untranslated region of VEGF mRNA, thereby decreasing its stability. This suggests that TIS11B is a potential target in antiangiogenic therapy. In addition, TIS11B may also be an important regulator of myogenesis, as its expression is upregulated during murine myoblast differentiation.Synonyms: BERG36, BRF1, Butyrate response factor 1, EGF-response factor 1, ERF-1, ERF1, RNF162B, Tetradecanoyl phorbol acetate-inducible sequence 11b