PRKCSH 抗体
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- 抗原 See all PRKCSH 抗体
- PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRKCSH antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- This antibody detects endogenous levels of Glucosidase II beta protein.(region surrounding Lys117)
- 纯化方法
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product PRKCSH Primary Antibody
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anti-Protein Kinase C Substrate 80K-H (PRKCSH) antibody
KO Validated PRKCSH 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (Internal Region) antibodyPRKCSH 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) antibodyPRKCSH 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (AA 15-302) antibodyPRKCSH 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (AA 1-398) antibodyPRKCSH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (AA 1-398) antibodyPRKCSH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (AA 155-204) antibodyPRKCSH 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (AA 268-377) antibodyPRKCSH 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3H7 unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) antibodyPRKCSH 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Protein Kinase C Substrate 80K-H (PRKCSH) (AA 81-130) antibodyPRKCSH 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))
- 别名
- Glucosidase 2 Subunit beta (PRKCSH 产品)
- 别名
- AGE-R2 antibody, G19P1 antibody, PCLD antibody, PKCSH antibody, PLD1 antibody, 80K-H antibody, VASAP-60 antibody, zgc:55653 antibody, prkcsh antibody, MGC75615 antibody, MGC82696 antibody, PRKCSH antibody, protein kinase C substrate 80K-H antibody, protein kinase C substrate 80K-H L homeolog antibody, phospholipase D1 antibody, PRKCSH antibody, Prkcsh antibody, prkcsh antibody, prkcsh.L antibody, Bm1_42055 antibody, LOC100304710 antibody, LOC100526729 antibody, PLD1 antibody
- 背景
- Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains alpha and beta subunits (Glucosidase IIalpha and Glucosidase IIbeta). The alpha and beta subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIalpha is the catalyitc core of the enzyme and can function independently of the beta subunit. The sequence of Glucosidase IIbeta encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIbeta is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIbeta was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIbeta retains the complete complex in the ER.Synonyms: 80K-H protein, G19P1, Glucosidase II beta, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain
- 分子量
- approx. 59 kDa
- 基因ID
- 5589
- NCBI登录号
- NP_001001329
- UniProt
- P14314
- 途径
- Cellular Glucan Metabolic Process, Methionine Biosynthetic Process
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