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COL18A1 抗体

COL18A1 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN307188
发货至: 中国
  • 抗原 See all COL18A1 抗体
    COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
    适用
    • 77
    • 48
    • 23
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 77
    • 9
    • 2
    小鼠
    克隆类型
    • 78
    • 11
    单克隆
    标记
    • 45
    • 9
    • 7
    • 7
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This COL18A1 antibody is un-conjugated
    应用范围
    • 75
    • 49
    • 32
    • 15
    • 13
    • 13
    • 6
    • 6
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
    纯化方法
    Thiophilic adsorption
    免疫原
    Full-length recombinant murine endostatin.
    亚型
    IgG
    Top Product
    Discover our top product COL18A1 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (1:100), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50 ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    50 mM sodium phosphate,  pH 8.0, 0.05 % sodium azide, before the addition of glycerol to 30 %. Sourced in Ascites.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
    别名
    COL18A1 / Endostatin (COL18A1 产品)
    别名
    KNO antibody, KNO1 antibody, KS antibody, cb371 antibody, cb529 antibody, fc96d06 antibody, sb:cb529 antibody, wu:fb47h10 antibody, wu:fb77g12 antibody, wu:fc50f04 antibody, wu:fc96d06 antibody, wu:fk02h12 antibody, kno antibody, kno1 antibody, collagen type XVIII alpha 1 chain antibody, collagen type XVIII alpha 1 chain a antibody, collagen, type XVIII, alpha 1 antibody, collagen type XVIII alpha 1 antibody, COL18A1 antibody, col18a1a antibody, Col18a1 antibody, col18a1 antibody
    物质类
    Chemical
    背景
    Name/Gene ID: COL18A1
    Family: Collagen

    Synonyms: COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1
    基因ID
    80781
    UniProt
    P39060
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