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HES1 抗体 (AA 24-35)

This anti-HES1 antibody is a 兔 多克隆 antibody detecting HES1 in WB, IHC, IF, IHC (p) 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 马, 猴, Bat, 非洲爪蟾 和 Hamster. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN307170
发货至: 中国

Quick Overview for HES1 抗体 (AA 24-35) (ABIN307170)

抗原

See all HES1 抗体
HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

适用

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人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 马, 猴, Bat, 非洲爪蟾, Hamster

宿主

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克隆类型

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多克隆

标记

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This HES1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    AA 24-35

    品牌

    IHC-plus™

    特异性

    Recognizes human HES-1 (Hairy family).

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%) Newt, Smelt (91%) Chicken (82%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide corresponding to aa24-35 (TPDKPKTASEH) OF HES1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%), Newt, Smelt (91%), Chicken (82%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:200 - 1:1000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Human neural stem cells, Raji cell lysate.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2. No preservative added.

    储存液

    Without preservative

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Moya, Umans, Maas, Pereira, Beets, Francis, Sents, Robertson, Mummery, Huylebroeck, Zwijsen: "Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades." in: Developmental cell, Vol. 22, Issue 3, pp. 501-14, (2012) (PubMed).

  • 抗原

    HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

    别名

    HES1 / HES-1

    背景

    Name/Gene ID: HES1

    Synonyms: HES1, BHLHb39, HHL, HL, Hairy and enhancer of split 1, Hairy homolog, HRY, Hairy homolog (Drosophila), Transcription factor HES-1, Hairy-like protein, HES-1

    基因ID

    3280

    UniProt

    Q14469

    途径

    DNA Damage Repair
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