Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
交叉反应
人
产品特性
CYPIVF11, EC 1.14.14.1,The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP4F11 is an isoform of the cytochrome P450 4F protein and is expressed mainly in human liver, followed by kidney, heart, and skeletal muscle. CYP4F11 contains 524 amino acid residues that share 80.0, 82.3 and 79.2 % identity to CYP4F2, CYP4F3, and CYP4F8 amino acid sequences, respectively. CYP4F11 has catalytic properties towards endogenous eicosanoids as well as some clinically relevant drugs, and is able to metabolize large molecules such as erythromycin.
纯化方法
Protein A/G Chromatography
免疫原
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human CYP450 4F11 protein and mouse myeloma Ag8563 cells.
CYP4F11
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 555
应用备注
Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions. For optimal results, formalin fixed, paraffin embedded tissues need an antigen retreival by heat treatment in citrate buffer pH 6.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
抗原
CYP4F11
(Cytochrome P450, Family 4, Subfamily F, Polypeptide 11 (CYP4F11))