Kein 14 (C-Term) 抗体
适用: 人
IHC (fro), WB
宿主: 小鼠
Monoclonal
DE-SPK14
unconjugated
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- 抗原
- Kein 14
- 抗原表位
- C-Term
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
- 交叉反应
- 人
- 产品特性
- Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. This antibody reacts specifically with human keratin 14 by immunoblotting. In tissue sections, it provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.
- 纯化方法
- Protein A/G Chromatography
- 免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) and mouse myeloma cells.
- 克隆位点
- DE-SPK14
- 亚型
- IgG2b
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- 应用备注
- Detects human cytokeratin 14 by Western blot at 50 kDa. Does not react with other cytokeratins or other proteins. Also works for immunohistochemistry using frozen tissues. Does not work with paraffin embedded tissues. Optimal concentration should be evaluated by serial dilutions.
- 限制
- 仅限研究用
-
- 缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- 抗原
- Kein 14
- UniProt
- P02533
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