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Cytochrome C 抗体 (AA 1-80)

The 小鼠 单克隆 anti-Cytochrome C antibody (Clone 7H8-2C12) (ABIN305748) specifically detects Cytochrome C in WB, IHC, ICC 和 IHC (p). The antibody is reactive with 人, 大鼠, 小鼠, 马, Pigeon, Drosophila melanogaster 和 Hamster samples.
产品编号 ABIN305748
发货至: 中国
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Quick Overview for Cytochrome C 抗体 (AA 1-80) (ABIN305748)

抗原

See all Cytochrome C (CYCS) 抗体
Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

适用

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人, 大鼠, 小鼠, 马, Pigeon, Drosophila melanogaster, Hamster

宿主

  • 120
  • 120
  • 6
  • 1
小鼠

克隆类型

  • 164
  • 82
  • 1
单克隆

标记

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This Cytochrome C antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

7H8-2C12
  • 抗原表位

    • 41
    • 16
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
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    • 1
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    • 1
    • 1
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    • 1
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    • 1
    AA 1-80

    特异性

    An approx. 15 kD band is observed. This recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached).

    纯化方法

    Protein G purified

    免疫原

    Synthetic peptide from pigeon CYCS / Cytochrome c.

    Type of Immunogen: Synthetic peptide

    亚型

    IgG2b
  • 应用备注

    Approved: ICC, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Pigeon

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.05 % BSA, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

    别名

    CYCS / Cytochrome c

    背景

    Name/Gene ID: CYCS

    Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

    基因ID

    54205

    UniProt

    P99999

    途径

    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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