Adenosine A2a Receptor 抗体 (3rd Intracellular Domain)
Quick Overview for Adenosine A2a Receptor 抗体 (3rd Intracellular Domain) (ABIN305729)
抗原
See all Adenosine A2a Receptor (ADORA2A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- 3rd Intracellular Domain
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品牌
- IHC-plus™
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特异性
- Recognizes human adenosine receptor A2A at ~43kD.
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纯化方法
- Protein G purified
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免疫原
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6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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应用备注
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Approved: Flo, IHC, IHC-P (5 μg/mL), IHC-Pfa (1 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Adenosine A2a Receptor (ADORA2A)
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别名
- ADORA2A/Adenosine A2A Receptor
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背景
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Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR
Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8 -
基因ID
- 135
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UniProt
- P29274
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途径
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
抗原
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