ITGA4 抗体 (FITC)
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- 抗原 See all ITGA4 抗体
- ITGA4 (Integrin alpha 4 (ITGA4))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This ITGA4 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Anti-Mouse CD49d is recommended for use in flow cytometry for identification of .
- 免疫原
- AKR/Cum mouse spontaneous T lymphoma line TK1 Species
- 克隆位点
- R1-2
- 亚型
- IgG2b
- Top Product
- Discover our top product ITGA4 Primary Antibody
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anti-Integrin alpha 4 (ITGA4) (Internal Region) antibody
ITGA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 551-650) antibodyITGA4 适用: 人, 小鼠, 兔 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 833-977) antibodyITGA4 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7E4C5 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 376-558) antibodyITGA4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (C-Term) antibodyITGA4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, EIA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal R1-2 unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal DATK32 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 1 µg/10^6 cells
- 说明
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Fluorescein Isothiocyanate, FITC (Abs/Em: 495/ 517 nm).
- 试剂准备
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- Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD49d FITC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- ITGA4 (Integrin alpha 4 (ITGA4))
- 别名
- CD49d (ITGA4 产品)
- 别名
- CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody
- 背景
- CD49d is a 150 kDa glycoprotein, also known as alpha4 integrin or VLA-4 alpha chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells.
- 途径
- Integrin Complex
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