H-2Dd 抗体 (FITC)
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- 抗原 See all H-2Dd products
- H-2Dd (MHC Class I H-2Dd)
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This H-2Dd antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- The 34-2-12 monoclonal antibody reacts with the mouse alpha3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype.
- 免疫原
- C57BL/6 x DBA/2)F1 mouse splenocytes
- 克隆位点
- 34-2-12
- 亚型
- IgG2a
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anti-MHC Class I H-2Dd antibody
适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S unconjugated
anti-MHC Class I H-2Dd antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S PE
anti-MHC Class I H-2Dd antibody (Biotin)适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S Biotin
anti-MHC Class I H-2Dd antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S FITC
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- 应用备注
- It is recommended for use in flow cytometry. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent iless or equal to 0.25 µg per 10^6 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- 说明
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Fluorescein isothiocyanate (FITC). Abs/Em: 494/519 nm.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- H-2Dd (MHC Class I H-2Dd)
- 别名
- H-2Dd (H-2Dd 产品)
- 背景
- The 34-2-12 antibody (also known as 34-2-12S) recognizes the alpha3 domain of the H-2D^[d]. The binding of the antibody to its epitope is independent of the alpha1 and alpha2 domains and beta2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that the alpha3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].
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