Sca-1/Ly-6A/E 抗体 (FITC)
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- 抗原 See all Sca-1/Ly-6A/E (Ly6a) 抗体
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Sca-1/Ly-6A/E antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Rat Anti-Mouse Ly-6A/E is recommended for use in flow cytometry for identification of the Mouse Ly-6A/E.
- 免疫原
- IL-2-dependent mouse T-cell line CTL-L
- 克隆位点
- D7
- 亚型
- IgG2a
- Top Product
- Discover our top product Ly6a Primary Antibody
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anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (APC)
Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 APC
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠, 大鼠, 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) (AA 91-108) antibodyLy6a 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (PE)Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 PE
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (Biotin)Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 Biotin
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (FITC)Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 FITC
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (SPRD)Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 SPRD
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. Recommeded Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometry analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal to 0.1 µg per 10^6 cells in 100 µL volume or 100 µL of whole bloodIt is recommended that the reagent be titrated for optimal performance for each application.
- 说明
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Fluorescein isothiocyanate (FITC). Abs/Em: 495/517 nm.
- 试剂准备
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- Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add antibody and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided Phosphate-buffered solution, pH 7.2, containing 0.09% Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
- 别名
- Ly6 A/E (Ly6a 产品)
- 别名
- Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody
- 背景
- Strains expressing the Ly6.1 haplotype have low Ly6A/E expression on resting peripheral lymphocytes. The expression of Ly6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly6A/E is thought to be involved in the regulation of both T and B cell responses.
- 途径
- Sensory Perception of Sound, Activated T Cell Proliferation
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