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CD14 抗体 (AA 65-327)

Cited in 4+ publications. This anti-CD14 antibody is a 兔 多克隆 antibody detecting CD14 in WB 和 IHC. Suitable for 人.
产品编号 ABIN3044565
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD14 抗体 (AA 65-327) (ABIN3044565)

抗原

See all CD14 抗体
CD14

适用

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宿主

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克隆类型

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多克隆

标记

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This CD14 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    AA 65-327

    原理

    Anti-CD14 Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-CD14 Antibody (ABIN3044565). Tested in IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E. coli-derived human CD14 recombinant protein (Position: N65-D327). Human CD14 shares 69.8% and 66.8% amino acid (aa) sequence identity with mouse and rat CD14, respectively.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Wu, Yang, Qi, Zhou, Hu, Wang: "ANTI-INFLAMMATORY ACTIVITY OF PLATYCODIN D ON ALCOHOL-INDUCED FATTY LIVER RATS VIA TLR4-MYD88-NF-κB SIGNAL PATH." in: African journal of traditional, complementary, and alternative medicines : AJTCAM, Vol. 13, Issue 4, pp. 176-183, (2017) (PubMed).

    Wu, Liu, Zhang, Xing, Zheng, Ji: "Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 363-370, (2014) (PubMed).

    Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

    Zhao, Zhao, Feng, Zhang, Zhuang, Zhao, Li, Li, Liu: "Detection of vulnerable atherosclerotic plaque and prediction of thrombosis events in a rabbit model using 18F-FDG -PET/CT." in: PLoS ONE, Vol. 8, Issue 4, pp. e61140, (2013) (PubMed).

  • 抗原

    CD14

    别名

    CD14

    背景

    Synonyms: Monocyte differentiation antigen CD14 ,Myeloid cell-specific leucine-rich glycoprotein,CD14,Monocyte differentiation antigen CD14, urinary form,Monocyte differentiation antigen CD14, membrane-bound form,CD14,

    Tissue Specificity: Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages. .

    Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

    分子量

    50-55 kDa

    基因ID

    929

    UniProt

    P08571

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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