DIABLO 抗体 (AA 56-239)
(1 reference)-
- 抗原 See all DIABLO 抗体
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
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抗原表位
- AA 56-239
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DIABLO antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 原理
- Anti-Smac/Diablo Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-Smac/Diablo Antibody Picoband® (ABIN3044515). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.
- 亚型
- IgG
- Top Product
- Discover our top product DIABLO Primary Antibody
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anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 211-239), (C-Term) antibody
DIABLO 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20755 unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 131-239) antibodyDIABLO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 168-236) antibodyDIABLO 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 1-239) antibodyDIABLO 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (C-Term) antibodyDIABLO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 199-212) antibodyDIABLO 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 1-239) antibodyDIABLO 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 119-218) antibodyDIABLO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F9 unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (C-Term) antibodyDIABLO 适用: 人, Cow, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (C-Term) antibodyDIABLO 适用: 人, Cow, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: DIABLO diablo homolog (Drosophila)". 2. McNeish IA, Bell S, McKay T, Tenev T, Marani M, Lemoine NR (Jun 2003). "Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway". Experimental Cell Research 286 (2): 186-98. 3. Yu J, Wang P, Ming L, Wood MA, Zhang L (Jun 2007). "SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events". Oncogene 26 (29): 4189-98. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Genistein protects against Aβ25-35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers." in: BMC neuroscience, Vol. 18, Issue 1, pp. 12, (2017) (PubMed).
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: "Genistein protects against Aβ25-35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers." in: BMC neuroscience, Vol. 18, Issue 1, pp. 12, (2017) (PubMed).
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- 抗原
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 别名
- DIABLO (DIABLO 产品)
- 背景
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Synonyms: Diablo homolog, mitochondrial; IAP-binding protein with low pI;Second mitochondria-derived activator of caspase;Smac;DIABLO;SMAC;
Tissue Specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .
Background: Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO ( IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
- 分子量
- 25 kDa
- 基因ID
- 56616
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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