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ALPL 抗体 (N-Term)

Name: ALPL 抗体 (N-Term)
SKU: ABIN3044314
Availability: OutOfStock

ALPL 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Anti-Alkaline Phosphatase antibody, IHC(P) IHC(P): Rat Small Intestine Tissue

IHC

Western Blotting (WB) image for anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody (ABIN3044314)

4 images

(21 references)

产品编号 ABIN3044314

发货至: 中国

Datasheet as PDF

抗原 See all ALPL 抗体

ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
抗原表位
16

9

9

5

5

4

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-35, N-Term
适用
79

50

16

5

1

1

1

1

1
人, 大鼠
宿主
76

19

1

1
兔
Compatible Secondaries
克隆类型
77

20
多克隆
标记
60

9

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This ALPL antibody is un-conjugated
应用范围
69

38

26

17

13

13

13

12

11

8

5

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

序列

EKEKDPKYWR DQAQE

交叉反应（详细）

Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

产品特性

Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: alkaline phosphatase, liver/bone/kidney
Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme(AP-TNAP/TNSALP)

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase(21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.

亚型

IgG

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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody
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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody
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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (Internal Region) antibody
ALPL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 20-270) antibody
ALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody
ALPL 适用: 人 WB, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 2F4 unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 52-150) antibody
ALPL 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 18-335) antibody
ALPL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

有效期

12 months
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抗原

ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

别名

ALPL (ALPL 产品)

别名

ALP antibody, iap antibody, alpl antibody, AP-TNAP antibody, APTNAP antibody, HOPS antibody, TNAP antibody, TNSALP antibody, Akp-2 antibody, Akp2 antibody, PHOA antibody, TNS-AP antibody, alkaline phosphatase, liver/bone/kidney L homeolog antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase, intestinal, gene 2 antibody, alkaline phosphatase, liver/bone/kidney pseudogene antibody, alpl.L antibody, ALPL antibody, alpi.2 antibody, LOC100031702 antibody, Alpl antibody

背景

Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Synonyms: Akp2 antibody|Alkaline phosphatase intestinal antibody|Alkaline phosphatase intestinal precursor antibody|Alkaline phosphatase liver/bone/kidney antibody|Alkaline phosphatase placental antibody|Alkaline phosphatase placental like 2 antibody|Alkaline phosphatase placental like antibody|Alkaline phosphatase placental type antibody|Alkaline phosphomonoesterase antibody|ALP 1 antibody|ALP antibody|ALP I antibody|ALP L antibody|ALP P antibody|ALPG antibody|ALPI antibody|ALPL antibody|ALPP antibody|ALPPL antibody|ALPPL2 antibody|AP TNAP antibody|FLJ40094 antibody|GCAP antibody|Germ cell alkaline phosphatase antibody|Glycerophosphatase antibody|HOPS antibody|IAP antibody|Intestinal alkaline phosphatase antibody|Kasahara isozyme antibody|Nagao isozyme antibody|OTTHUMP00000164357 antibody|Placental alkaline phosphatase antibody|Placental like alkaline phosphatase antibody|PLAP 1 antibody|PLAP antibody|PLAP like antibody|Regan isozyme antibody|Testicular and thymus alkaline phosphatase antibody|Tissue non specific alkaline phosphatase antibody|Tissue nonspecific ALP antibody|TNAP antibody|TNSALP antibody

UniProt

P05186