NDUFA1 抗体 (C-Term)
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- 抗原 See all NDUFA1 抗体
- NDUFA1 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1))
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抗原表位
- AA 54-70, C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NDUFA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1(NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- 序列
- ISGVDRYYVS KGLENID
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1(NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: NADH dehydrogenase(ubiquinone) 1 alpha subcomplex, 1, 7.5 kDa
Protein Name: NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product NDUFA1 Primary Antibody
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anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) antibody
NDUFA1 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) (AA 1-70) antibodyNDUFA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) antibodyNDUFA1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) (AA 31-70) antibodyNDUFA1 适用: 小鼠 ELISA, IHC (p), IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) (AA 26-59) antibodyNDUFA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB53484 unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) (AA 24-70) antibodyNDUFA1 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 3B9-1A1 unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) antibody (HRP)NDUFA1 适用: 人 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) antibody (Biotin)NDUFA1 适用: 人 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) antibody (FITC)NDUFA1 适用: 人 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
anti-NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) (Internal Region) antibodyNDUFA1 适用: 人, 小鼠, 大鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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- 抗原
- NDUFA1 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1))
- 别名
- NDUFA1 (NDUFA1 产品)
- 别名
- 1810049F12Rik antibody, MWFE antibody, RGD1560955 antibody, CI-MWFE antibody, ZNF183 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 antibody, NADH:ubiquinone oxidoreductase subunit A1 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa antibody, Ndufa1 antibody, NDUFA1 antibody
- 背景
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NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100 % of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Synonyms: CI MWFE antibody|CI-MWFE antibody|Complex I MWFE antibody|complex I MWFE subunit antibody|Complex I-MWFE antibody|MWFE antibody|MWFE, B. taurus, homolog of antibody|NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 7.5 kDa antibody|NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 antibody|NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 antibody|NADH oxidoreductase subunit MWFE antibody|NADH ubiquinone oxidoreductase(complex 1) antibody|NADH ubiquinone oxidoreductase MWFE subunit antibody|NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1 antibody|NADH-ubiquinone oxidoreductase MWFE subunit antibody|NDUA1_HUMAN antibody|NDUFA 1 antibody|NDUFA1 antibody|Type I dehydrogenase antibody|ZNF 183 antibody|ZNF183 antibody - UniProt
- O15239
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