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SQSTM1 抗体 (N-Term)

Name: SQSTM1 抗体 (N-Term)
SKU: ABIN3044026
Availability: OutOfStock

SQSTM1 适用: 人, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Anti-SQSTM1/p62 antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: HELA Cell Lysate Lane 3: U87 Cell Lysate Lane 4: A549 Cell Lysate

Anti-SQSTM1/p62 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue

IHC

Anti-SQSTM1/p62 antibody, ICC ICC: Hela Cell

IHC

3 images

(4 references)

产品编号 ABIN3044026

发货至: 中国

Datasheet as PDF

抗原 See all SQSTM1 抗体

SQSTM1 (Sequestosome 1 (SQSTM1))
抗原表位
19

17

15

13

8

7

7

7

7

6

6

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 91-110, N-Term
适用
154

64

60

18

4

4

4

4

3

2

2

2

1

1

1

1
人, 大鼠
宿主
128

24

1

1
兔
Compatible Secondaries
克隆类型
127

27
多克隆
标记
75

11

10

7

7

6

5

5

5

5

5

5

1

1

1

1

1

1

1

1
This SQSTM1 antibody is un-conjugated
应用范围
104

58

41

40

22

18

16

15

14

13

10

5

3

2

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

原理

Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

序列

KDDIFRIYIK EKKECRRDHR

交叉反应（详细）

Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

产品特性

Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: sequestosome 1
Protein Name: Sequestosome-1

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1(91-110aa KDDIFRIYIKEKKECRRDHR), different from the related rat and mouse sequences by one amino acid.

亚型

IgG

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SQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
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SQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated

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SQSTM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (C-Term) antibody
SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
SQSTM1 适用: 人 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 4E2 unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
SQSTM1 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A2 unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

有效期

12 months
Yu, Li, Wang, He, Ding, Zhang, Yu, Shi, Cui, Wang, Wang, Sun, Zhang, Du, Zhu: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).

Jeong, He, Nohara, Park, Shin, Kim, Shimomura, Koike, Yoo, Chen: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." in: Scientific reports, Vol. 5, pp. 12801, (2017) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Li, Jen, Wu, Lee, Fang, Quigley, Lee, Wang, Zhou, Vergnes, Chen, Li, Reue, Ann, Hsiai: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." in: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, (2016) (PubMed).
抗原

SQSTM1 (Sequestosome 1 (SQSTM1))

别名

SQSTM1 (SQSTM1 产品)

别名

SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody

背景

SQSTM1(Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2(SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al.(1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al.(1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Synonyms: A170 antibody|EBI 3 associated protein of 60 kDa antibody|EBI 3 associated protein p60 antibody|EBI3 associated protein of 60 kDa antibody|EBI3 associated protein p60 antibody|EBI3-associated protein of 60 kDa antibody|EBIAP antibody|MGC127197 antibody|ORCA antibody|OSF-6 antibody|Osi antibody|OSIL antibody|Oxidative stress induced like antibody|p60 antibody|p62 antibody|p62B antibody|Paget disease of bone 3 antibody|PDB 3 antibody|PDB3 antibody|Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody|Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody|Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody|PKC-zeta-interacting protein antibody|Protein kinase C-zeta-interacting protein antibody|Sequestosome 1 antibody|Sequestosome-1 antibody|SQSTM 1 antibody|SQSTM_HUMAN antibody|Sqstm1 antibody|STAP antibody|STONE14 antibody|Ubiquitin binding protein p62 antibody|Ubiquitin-binding protein p62 antibody|ZIP 3 antibody|ZIP antibody|ZIP3 antibody

UniProt

Q13501

途径

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy