电话:: +86 (0512) 65829739
传真:: +86 (010) 6788 5057
电子邮件:: orders@antibodies-online.cn

我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

SQSTM1 抗体 (N-Term)

SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) (SQSTM1 抗体 (N-Term))

Anti- SQSTM1 Picoband antibody,IHC(F) IHC(F): Rat Spleen Tissue (SQSTM1 抗体 (N-Term))

Anti- SQSTM1 Picoband antibody,IHC(F) IHC(F): Mouse Spleen Tissue (SQSTM1 抗体 (N-Term))

Anti- SQSTM1 Picoband antibody,IHC(P) IHC(P): Mouse Thymus Tissue (SQSTM1 抗体 (N-Term))

Anti- SQSTM1 Picoband antibody,IHC(P) IHC(P): Rat Spleen Tissue (SQSTM1 抗体 (N-Term))

Anti- SQSTM1 Picoband antibody,IHC(P) IHC(P): Human Lung Cancer Tissue (SQSTM1 抗体 (N-Term))

6 images

(5 references)

产品编号 ABIN3043937

发货至: 中国

Datasheet as PDF

抗原 See all SQSTM1 抗体

SQSTM1 (Sequestosome 1 (SQSTM1))
抗原表位
17

14

9

8

8

7

7

6

5

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 69-96, N-Term
适用
131

57

52

16

3

3

3

3

2

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
107

22

1

1
兔
Compatible Secondaries
克隆类型
105

26
多克隆
标记
73

9

8

5

4

4

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1
This SQSTM1 antibody is un-conjugated
应用范围
89

53

40

38

18

18

16

16

13

13

12

4

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

原理

Anti-SQSTM1/p62 Antibody Picoband®

序列

DEDGDLVAFS SDEELTMAMS YVKDDIFR

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-SQSTM1/p62 Antibody Picoband® (ABIN3043937). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62, identical to the related mouse and rat sequences.

亚型

IgG

Top Product

Discover our top product SQSTM1 Primary Antibody
anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IF, IP, FACS, IHC (p), ICC, PLA 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 51-150) antibody
SQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
SQSTM1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
SQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 232-356) antibody
SQSTM1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H7E2 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 317-346) antibody
SQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 136-400) antibody
SQSTM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (C-Term) antibody
SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
SQSTM1 适用: 人, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 5A2 unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 2 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry, 0.5-1 μg/mL, Human1. Hocking, L. J., Lucas, G. J. A., Daroszewska, A., Mangion, J., Olavesen, M., Cundy, T., Nicholson, G. C., Ward, L., Bennett, S. T., Wuyts, W., Van Hul, W., Ralston, S. H. Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease. Hum. Molec. Genet. 11: 2735-2739, 2002. 2. Joung, I., Strominger, J. L., Shin, J. Molecular cloning of a phosphotyrosine-independent ligand of the p56-lck SH2 domain. Proc. Nat. Acad. Sci. 93: 5991-5995, 1996. 3. Park, I., Chung, J., Walsh, C. T., Yun, Y., Strominger, J. L., Shin, J. Phosphotyrosine-independent binding of a 62- kDa protein to the src homology 2 (SH2) domain of p56-lck and its regulation by phosphorylation of ser-59 in the lck unique N-terminal region. Proc. Nat. Acad. Sci. 92: 12338-12342, 1995.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Yu, Li, Wang, He, Ding, Zhang, Yu, Shi, Cui, Wang, Wang, Sun, Zhang, Du, Zhu: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).

Song, Hu, Li, Zheng, Wang, Guo, Shi, Liu: "Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication." in: Archives of virology, Vol. 163, Issue 1, pp. 135-144, (2018) (PubMed).

Jeong, He, Nohara, Park, Shin, Kim, Shimomura, Koike, Yoo, Chen: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." in: Scientific reports, Vol. 5, pp. 12801, (2017) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Li, Jen, Wu, Lee, Fang, Quigley, Lee, Wang, Zhou, Vergnes, Chen, Li, Reue, Ann, Hsiai: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." in: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, (2016) (PubMed).
抗原

SQSTM1 (Sequestosome 1 (SQSTM1))

别名

SQSTM1 (SQSTM1 产品)

背景

Synonyms: Sequestosome-1,EBI3-associated protein of 60 kDa,EBIAP,p60,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,Ubiquitin-binding protein p62,SQSTM1,ORCA, OSIL,
Tissue Specificity: Ubiquitously expressed. .
Background: SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
Sequence Similarities: Contains 1 PB1 domain.

分子量

62 kDa

基因ID

8878

UniProt

Q13501

途径

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

You are here: