SQSTM1 抗体 (N-Term)
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北京 101111
Quick Overview for SQSTM1 抗体 (N-Term) (ABIN3043937)
抗原
See all SQSTM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 69-96, N-Term
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原理
- Anti-SQSTM1/p62 Antibody
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序列
- DEDGDLVAFS SDEELTMAMS YVKDDIFR
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-SQSTM1/p62 Antibody (ABIN3043937). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IF, ICC, IP, FACS, IHC (p), PLA 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 51-150) antibodySQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibodySQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibodySQSTM1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 232-356) antibodySQSTM1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H7E2 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 317-346) antibodySQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 136-400) antibodySQSTM1 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (C-Term) antibodySQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) antibodySQSTM1 适用: 人, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 317-346) antibodySQSTM1 适用: 人, Green Monkey WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 2 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry, 0.5-1 μg/mL, Human -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).
: "Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication." in: Archives of virology, Vol. 163, Issue 1, pp. 135-144, (2018) (PubMed).
: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." in: Scientific reports, Vol. 5, pp. 12801, (2017) (PubMed).
: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).
: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." in: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, (2016) (PubMed).
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: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).
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- SQSTM1 (Sequestosome 1 (SQSTM1))
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别名
- SQSTM1
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背景
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Synonyms: Sequestosome-1,EBI3-associated protein of 60 kDa,EBIAP,p60,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,Ubiquitin-binding protein p62,SQSTM1,ORCA, OSIL,
Tissue Specificity: Ubiquitously expressed. .
Background: SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
Sequence Similarities: Contains 1 PB1 domain.
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分子量
- 62 kDa
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基因ID
- 8878
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UniProt
- Q13501
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途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
抗原
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