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MCL-1 抗体 (AA 1-350)

MCL1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3043876
发货至: 中国
  • 抗原 See all MCL-1 (MCL1) 抗体
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    抗原表位
    • 25
    • 16
    • 15
    • 11
    • 8
    • 6
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    • 1
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    • 1
    • 1
    • 1
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    • 1
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    AA 1-350
    适用
    • 126
    • 54
    • 53
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 119
    • 11
    • 1
    • 1
    克隆类型
    • 112
    • 22
    多克隆
    标记
    • 64
    • 9
    • 9
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
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    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MCL-1 antibody is un-conjugated
    应用范围
    • 102
    • 54
    • 46
    • 36
    • 25
    • 25
    • 20
    • 19
    • 13
    • 9
    • 7
    • 4
    • 3
    Western Blotting (WB), Immunohistochemistry (IHC)
    原理
    Anti-MCL1 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-MCL1 Antibody Picoband® (ABIN3043876). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.
    亚型
    IgG
    Top Product
    Discover our top product MCL1 Primary Antibody
  • 应用备注
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    1. Wertz, I. E., Kusam, S., Lam, C., Okamoto, T., Sandoval, W., Anderson, D. J., Helgason, E., Ernst, J. A., Eby, M., Liu, J., Belmont, L. D., Kaminker, J. S., and 15 others. Sensitivity to antitubulin chemotherapeutics is regulated by MCL1 and FBW7. Nature 471: 110-114, 2011. Note: Erratum: Nature 475: 122 only, 2011. 2. Kozopas KM, Yang T, Buchan HL, Zhou P, Craig RW (May 1993). "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2". Proc Natl Acad Sci U S A 90 (8): 3516-20. 3. Craig RW, Jabs EW, Zhou P, Kozopas KM, Hawkins AL, Rochelle JM, Seldin MF, Griffin CA (February 1995). "Human and mouse chromosomal mapping of the myeloid cell leukemia-1 gene: MCL1 maps to human chromosome 1q21, a region that is frequently altered in preneoplastic and neoplastic disease". Genomics 23 (2): 457-63. 4. "Entrez Gene: MCL1 myeloid cell leukemia sequence 1 (BCL2-related)"
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Liu, Chen, Wang, Yang, Xue, Zhu: "Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells." in: FEBS letters, Vol. 589, Issue 8, pp. 897-903, (2015) (PubMed).

  • 抗原
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    别名
    MCL1 (MCL1 产品)
    背景

    Synonyms: Induced myeloid leukemia cell differentiation protein Mcl-1,Bcl-2-like protein 3,Bcl2-L-3,Bcl-2-related protein EAT/mcl1,mcl1/EAT,MCL1,BCL2L3,

    Tissue Specificity: Ubiquitously expressed, with more abundant expression in the brain.

    Background: MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 s its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.

    Sequence Similarities: Belongs to the Bcl-2 family.

    分子量
    37 kDa
    基因ID
    4170
    UniProt
    Q07820
    途径
    MAPK Pathway
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