MAP2K7 抗体 (N-Term)
Quick Overview for MAP2K7 抗体 (N-Term) (ABIN3043875)
抗原
See all MAP2K7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-40, N-Term
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原理
- Anti-MEK7/MAP2K7 Antibody Picoband®
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序列
- AASSLEQKLS RLEAKLKQEN REARRRIDLN LDISPQRPR
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MEK7/MAP2K7 Antibody Picoband® (ABIN3043875). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) antibody
MAP2K7 适用: 人 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4E5 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (AA 1-250) antibodyMAP2K7 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (AA 1-99) antibodyMAP2K7 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (AA 113-388) antibodyMAP2K7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (AA 17-160) antibodyMAP2K7 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (Internal Region) antibodyMAP2K7 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) antibodyMAP2K7 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (AA 206-233) antibodyMAP2K7 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (pSer271), (pThr275) antibodyMAP2K7 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) (AA 363-396) antibodyMAP2K7 适用: 人 WB 宿主: 兔 Polyclonal RB53024 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Lu, X., Nemoto, S., Lin, A. Identification of c-jun NH(2)-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38. J. Biol. Chem. 272: 24751-24754, 1997. 2. Schramek, D., Kotsinas, A., Meixner, A., Wada, T., Elling, U., Pospisilik, J. A., Neely, G. G., Zwick, R.-H., Sigl, V., Forni, G., Serrano, M., Gorgoulis, V. G., Penninger, J. M. The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression. Nature Genet. 43: 212-219, 2011. 3. Wu, Z., Wu, J., Jacinto, E., Karin, M. Molecular cloning and characterization of human JNKK2, a novel jun NH(2)-terminal kinase-specific kinase. Molec. Cell. Biol. 17: 7407-7416, 1997. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))
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别名
- MAP2K7
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背景
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Synonyms: Dual specificity mitogen-activated protein kinase kinase 7;MAP kinase kinase 7;MAPKK 7;2.7.12.2;JNK-activating kinase 2;MAPK/ERK kinase 7;MEK 7;Stress-activated protein kinase kinase 4;SAPK kinase 4;SAPKK-4;SAPKK4;c-Jun N-terminal kinase kinase 2;JNK kinase 2;JNKK 2;MAP2K7;JNKK2, MEK7, MKK7, PRKMK7, SKK4;
Tissue Specificity: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. .
Background: MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The MKK7 protein exists as six different isoforms with three possible N-termini (α, β, and γ isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 exerts its tumor suppressive function through p53.
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分子量
- 47 kDa
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基因ID
- 5609
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UniProt
- O14733
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途径
- MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
抗原
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