CTCF 抗体 (AA 521-727)
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北京 101111
Quick Overview for CTCF 抗体 (AA 521-727) (ABIN3043817)
抗原
See all CTCF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 521-727
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原理
- Anti-CTCF Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-CTCF Antibody (ABIN3043817). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CTCF recombinant protein (Position: K521-R727). Human CTCF shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat CTCF, respectively.
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亚型
- IgG
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anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-260) antibody
CTCF 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibodyCTCF 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibodyCTCF 适用: 人 WB, ChIP-seq, CUT&RUN 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (C-Term) antibodyCTCF 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-260) antibodyCTCF 适用: 人 WB, IHC, ELISA, ChIP 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 334-364) antibodyCTCF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB53773 unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (N-Term) antibodyCTCF 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 猴, 豚鼠, Bat, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibodyKD Validated CTCF 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3520 unconjugated Recombinant Antibody
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 451-550) antibodyCTCF 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-100) antibodyCTCF 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))
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别名
- CTCF
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背景
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Synonyms: Transcriptional repressor CTCF,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog,CTCF,
Tissue Specificity: Ubiquitous. Absent in primary spermatocytes. .
Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. And this nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Sequence Similarities: Belongs to the CTCF zinc-finger protein family.
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分子量
- 150 kDa
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基因ID
- 10664
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UniProt
- P49711
抗原
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