我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

NLRP3 抗体 (AA 21-194)

Name: NLRP3 抗体 (AA 21-194)
SKU: ABIN3043571
Availability: OutOfStock

NLRP3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

Observed bind size: 118KD (NLRP3 抗体 (AA 21-194))

Anti-CIAS1/NALP3 Picoband antibody, All lanes: Anti CIAS1 at 0. (NLRP3 抗体 (AA 21-194))

2 images

(3 references)

产品编号 ABIN3043571

发货至: 中国

Datasheet as PDF

抗原 See all NLRP3 抗体

NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
抗原表位
10

8

6

6

5

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-194
适用
72

30

17

6

2

1

1

1

1
人
宿主
69

12

2

1
兔
Compatible Secondaries
克隆类型
63

21
多克隆
标记
51

8

5

5

2

2

2

2

2

2

2

1
This NLRP3 antibody is un-conjugated
应用范围
51

41

16

13

10

9

6

5

4

1

1

1

1

1

1

1

1
Western Blotting (WB)

原理

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® (ABIN3043571). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

亚型

IgG

Top Product

Discover our top product NLRP3 Primary Antibody
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-93), (Pyrin Domain) antibody
NLRP3 适用: 人, 小鼠 WB, IHC, IF, IP 宿主: 小鼠 Monoclonal Cryo-2 unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 15-120) antibody
NLRP3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, IHC (p), FACS, ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 671-1033) antibody
NLRP3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (C-Term) antibody
Verified NLRP3 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (C-Term) antibody
NLRP3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (N-Term) antibody
NLRP3 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 12-31), (N-Term) antibody
NLRP3 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 30-102) antibody
NLRP3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-100) antibody
NLRP3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B1 unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 65-91), (N-Term) antibody
NLRP3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB22404 unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human
1. Agostini, L., Martinon, F., Burns, K., McDermott, M. F., Hawkins, P. N., Tschopp, J. NALP3 forms an IL-1-beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. 2. Allen, I. C., Scull, M. A., Moore, C. B., Holl, E. K., McElvania-TeKippe, E., Taxman, D. J., Guthrie, E. H., Pickles, R. J., Ting, J. P.-Y. The NLRP3 inflammasome mediates in vivo innate immunity to influenza A virus through recognition of viral RNA. Immunity 30: 556-565, 2009. 3. Hoffman HM, Wright FA, Broide DH, Wanderer AA, Kolodner RD (Jun 2000). "Identification of a locus on chromosome 1q44 for familial cold urticaria". Am J Hum Genet 66 (5): 1693-8.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Zhang, Li, Xu, Xiang, Ma: "Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).

Xue, Wang, Du, Zhu: "Maternal obesity induces gut inflammation and impairs gut epithelial barrier function in nonobese diabetic mice." in: The Journal of nutritional biochemistry, Vol. 25, Issue 7, pp. 758-64, (2014) (PubMed).

Wang, Yang, Liang, Zhu, Du: "Grape seed extract prevents skeletal muscle wasting in interleukin 10 knockout mice." in: BMC complementary and alternative medicine, Vol. 14, pp. 162, (2014) (PubMed).
抗原

NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

别名

NLRP3 (NLRP3 产品)

背景

Synonyms: NACHT, LRR and PYD domains-containing protein 3,Angiotensin/vasopressin receptor AII/AVP-like,Caterpiller protein 1.1,CLR1.1,Cold autoinflammatory syndrome 1 protein,Cryopyrin,PYRIN-containing APAF1-like protein 1,NLRP3,C1orf7, CIAS1, NALP3, PYPAF1,
Tissue Specificity: Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. .
Background: NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. It is mapped to 1q44. The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, it has been showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.
Sequence Similarities: Belongs to the NLRP family.

分子量

118 kDa

基因ID

114548

UniProt

Q96P20

途径

Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome