KAP1 抗体 (AA 699-835)
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北京 101111
Quick Overview for KAP1 抗体 (AA 699-835) (ABIN3043491)
抗原
See all KAP1 (TRIM28) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 699-835
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原理
- Anti-KAP1/TRIM28 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-KAP1/TRIM28 Antibody (ABIN3043491). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.
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亚型
- IgG
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anti-Tripartite Motif Containing 28 (TRIM28) (AA 576-835) antibody
TRIM28 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibodyVerified TRIM28 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibodyTRIM28 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4E6 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 适用: 人 WB, IF, IP, ICC, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibodyTRIM28 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibodyTRIM28 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibodyTRIM28 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (pSer824) antibodyTRIM28 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
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别名
- TRIM28
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背景
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Synonyms: Transcription intermediary factor 1-beta,TIF1-beta,E3 SUMO-protein ligase TRIM28,6.3.2.-,KRAB-associated protein 1,KAP-1,KRAB-interacting protein 1,KRIP-1,Nuclear corepressor KAP-1,RING finger protein 96,Tripartite motif-containing protein 28,TRIM28,KAP1, RNF96, TIF1B,
Tissue Specificity: Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. .
Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
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分子量
- 100 kDa
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基因ID
- 10155
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UniProt
- Q13263
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途径
- Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
抗原
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