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TNF alpha 抗体 (AA 80-235)

Name: TNF alpha 抗体 (AA 80-235)
SKU: ABIN3042817
Availability: OutOfStock

TNF alpha 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of TNF alpha using anti- TNF alpha antibody . (TNF alpha 抗体 (AA 80-235))

1 image

(51 references)

产品编号 ABIN3042817

发货至: 中国

Datasheet as PDF

抗原 See all TNF alpha 抗体

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
抗原表位
42

26

14

14

14

9

8

5

5

5

5

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 80-235
适用
309

182

117

63

47

39

32

21

18

18

11

10

10

9

5

4

3

2

2

1

1

1

1

1

1
小鼠
宿主
244

176

27

22

7

3

2

1

1
兔
Compatible Secondaries
克隆类型
254

223
多克隆
标记
235

63

38

20

13

10

6

6

6

6

6

6

6

6

5

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This TNF alpha antibody is un-conjugated
应用范围
309

191

119

100

72

54

50

41

40

40

40

37

31

29

12

9

9

7

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

原理

Anti-TNF alpha Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-< a href="https://www.bosterbio.com/anti-tnf-alpha-picoband-trade-antibody-a00002-2-boster.html" style="color:#EA8D28">TNF alpha Antibody (ABIN3042817). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E. coli-derived mouse TNF alpha recombinant protein (Position: L80-L235).

亚型

IgG

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anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibody
TNF alpha 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibody
TNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)
TNF alpha 适用: 人, Pig, Non-Human Primate ICFC 宿主: 小鼠 Monoclonal MAb11 FITC

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibody
TNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated

应用备注

Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse
1. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984. 2. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide. Carrier free (No BSA)

储存液

Sodium azide, Without preservative

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
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抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF (TNF alpha 产品)

背景

Synonyms: Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Tumor necrosis factor, membrane form,N-terminal fragment,NTF,Intracellular domain 1,ICD1,Intracellular domain 2,ICD2,C-domain 1,C-domain 2,Tumor necrosis factor, soluble form,Tnf,Tnfa, Tnfsf2,
Tissue Specificity: Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles).
Background: TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

分子量

17 kDa

UniProt

P06804

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome