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TNF alpha 抗体 (AA 89-235)

Name: TNF alpha 抗体 (AA 89-235)
SKU: ABIN3042522
Availability: OutOfStock

TNF alpha 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated

Anti-TNF alpha Picoband antibody, All lanes: Anti-TNF alpha at 0. (TNF alpha 抗体 (AA 89-235))

1 image

(57 references)

产品编号 ABIN3042522

发货至: 中国

Datasheet as PDF

抗原 See all TNF alpha 抗体

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
抗原表位
49

39

20

14

14

9

8

8

7

7

5

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 89-235
适用
323

188

128

69

54

46

41

22

22

21

13

11

10

10

9

5

5

3

2

1

1

1

1

1

1
大鼠
宿主
285

212

27

22

7

6

2

1

1
兔
Compatible Secondaries
克隆类型
298

259
多克隆
标记
296

72

46

20

13

11

7

6

6

6

6

6

6

6

5

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This TNF alpha antibody is un-conjugated
应用范围
389

190

180

121

120

91

72

66

49

41

40

39

37

29

12

9

9

7

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1
Western Blotting (WB)

原理

Anti-TNF alpha Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-TNF alpha Antibody Picoband® (ABIN3042522). Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.

亚型

IgG

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TNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Rat
1. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985. 2. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF (TNF alpha 产品)

背景

Synonyms: Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Tumor necrosis factor, membrane form,N-terminal fragment,NTF,Intracellular domain 1,ICD1,Intracellular domain 2,ICD2,C-domain 1,C-domain 2,Tumor necrosis factor, soluble form,Tnf,Tnfa, Tnfsf2,
Tissue Specificity: Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine. .
Background: TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Sequence Similarities: Belongs to the nuclear hormone receptor family. NR3 subfamily.

分子量

26 kDa

UniProt

P16599

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome