电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

POLH 抗体 (AA 157-361)

POLH 适用: 人, 大鼠 WB, IF, IHC, ICC, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3042492
发货至: 中国
  • 抗原 See all POLH 抗体
    POLH (Polymerase (DNA Directed), eta (POLH))
    抗原表位
    • 14
    • 5
    • 3
    • 2
    • 2
    • 1
    AA 157-361
    适用
    • 40
    • 12
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠
    宿主
    • 38
    • 3
    克隆类型
    • 40
    • 1
    多克隆
    标记
    • 16
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLH antibody is un-conjugated
    应用范围
    • 28
    • 12
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    原理
    Anti-DNA polymerase eta/POLH Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins.
    产品特性
    Anti-DNA polymerase eta/POLH Antibody Picoband® (ABIN3042492). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human POLH recombinant protein (Position: A157-R361). Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.
    亚型
    IgG
    Top Product
    Discover our top product POLH Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. "Entrez Gene: POLH polymerase (DNA ed), eta". 2. Johnson RE, Kondratick CM, Prakash S, Prakash L (July 1999). "hRAD30 mutations in the variant form of xeroderma pigmentosum". Science 285 (5425): 263-5. 3. Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, Araki M, Iwai S, Takio K, Hanaoka F (June 1999). "The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta". Nature 399 (6737): 700-4.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    POLH (Polymerase (DNA Directed), eta (POLH))
    别名
    POLH (POLH 产品)
    背景

    Synonyms: DNA polymerase eta,2.7.7.7,RAD30 homolog A,Xeroderma pigmentosum variant type protein,POLH,RAD30, RAD30A, XPV,

    Tissue Specificity: Major component of lens fiber gap junctions.

    Background: DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-ed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

    分子量
    78 kDa
    基因ID
    5429
    UniProt
    Q9Y253
    途径
    DNA Damage Repair
You are here:
Chat with us!