POLH 抗体 (AA 157-361)
Quick Overview for POLH 抗体 (AA 157-361) (ABIN3042492)
抗原
See all POLH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 157-361
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原理
- Anti-DNA polymerase eta/POLH Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DNA polymerase eta/POLH Antibody Picoband® (ABIN3042492). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human POLH recombinant protein (Position: A157-R361). Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.
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亚型
- IgG
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anti-Polymerase (DNA Directed), eta (POLH) (AA 408-609) antibody
POLH 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) antibodyPOLH 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) antibodyPOLH 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 20-320) antibodyPOLH 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 1-713) antibodyPOLH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 1-713) antibodyPOLH 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (Internal Region) antibodyPOLH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) antibodyPOLH 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: POLH polymerase (DNA ed), eta". 2. Johnson RE, Kondratick CM, Prakash S, Prakash L (July 1999). "hRAD30 mutations in the variant form of xeroderma pigmentosum". Science 285 (5425): 263-5. 3. Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, Araki M, Iwai S, Takio K, Hanaoka F (June 1999). "The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta". Nature 399 (6737): 700-4. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- POLH (Polymerase (DNA Directed), eta (POLH))
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别名
- POLH
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背景
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Synonyms: DNA polymerase eta,2.7.7.7,RAD30 homolog A,Xeroderma pigmentosum variant type protein,POLH,RAD30, RAD30A, XPV,
Tissue Specificity: Major component of lens fiber gap junctions.
Background: DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-ed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.
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分子量
- 78 kDa
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基因ID
- 5429
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UniProt
- Q9Y253
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途径
- DNA Damage Repair
抗原
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