POLH 抗体 (Internal Region)
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- 抗原 See all POLH 抗体
- POLH (Polymerase (DNA Directed), eta (POLH))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLH antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- POLH Antibody detects endogenous levels of total POLH.
- 预测反应
- Sheep
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human POLH, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product POLH Primary Antibody
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anti-Polymerase (DNA Directed), eta (POLH) (AA 408-609) antibody
POLH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) antibodyPOLH 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) antibodyPOLH 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 20-320) antibodyPOLH 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 1-713) antibodyPOLH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 1-713) antibodyPOLH 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) (AA 157-361) antibodyPOLH 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), eta (POLH) antibodyPOLH 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- POLH (Polymerase (DNA Directed), eta (POLH))
- 别名
- POLH (POLH 产品)
- 别名
- POLH antibody, fc50a12 antibody, wu:fc50a12 antibody, zgc:136881 antibody, zgc:152642 antibody, RAD30 antibody, RAD30A antibody, XP-V antibody, XPV antibody, polymerase (DNA directed), eta S homeolog antibody, DNA polymerase eta antibody, polymerase (DNA directed), eta antibody, putative DNA polymerase eta antibody, polymerase (DNA directed), eta (RAD 30 related) antibody, polh.S antibody, POLH antibody, polh antibody, Tb10.70.6190 antibody, LINJ_21_0690 antibody, Polh antibody, LOC100443019 antibody
- 背景
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Description: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).
Gene: POLH
- 分子量
- 78kDa
- 基因ID
- 5429
- UniProt
- Q9Y253
- 途径
- DNA Damage Repair
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