HINT1 抗体 (Middle Region)
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- 抗原 See all HINT1 抗体
- HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))
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抗原表位
- AA 59-92, Middle Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HINT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB,IHC-P in Human,Mouse,Rat.
- 序列
- HISQISVAED DDESLLGHLM IVGKKCAADL GLNK
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB,IHC-P in Human,Mouse,Rat.
Gene Name: histidine triad nucleotide binding protein 1
Protein Name: Histidine triad nucleotide-binding protein 1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human HINT1 (59-92aa HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK), different from the related mouse and rat sequences by three amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product HINT1 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))
- 别名
- HINT1 (HINT1 产品)
- 别名
- hint antibody, pkci-1 antibody, prkcnh1 antibody, HINT antibody, NMAN antibody, PKCI-1 antibody, PRKCNH1 antibody, AA673479 antibody, Hint antibody, Ipk1 antibody, Pkci antibody, Prkci antibody, zgc:103764 antibody, CHPKCI antibody, HINTZ antibody, PKCIZ antibody, histidine triad nucleotide binding protein 1 antibody, histidine triad nucleotide binding protein 1 S homeolog antibody, histidine triad nucleotide binding protein 1-Z antibody, Histidine triad nucleotide-binding protein 1 antibody, HINT1 antibody, hint1 antibody, Hint1 antibody, hint1.S antibody, HINT1Z antibody, hint-1 antibody
- 背景
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Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/β-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.
Synonyms: HINT 1 antibody|HINT1 antibody|HINT1_HUMAN antibody|Histidine triad nucleotide binding protein 1 antibody|Histidine triad nucleotide-binding protein 1 antibody|PKCI 1 antibody|PKCI-1 antibody|PKCI1 antibody|PRKCNH1 antibody|Protein kinase C inhibitor 1 antibody| Protein kinase C interacting protein 1 antibody|Protein kinase C-interacting protein 1 antibody - 基因ID
- 3094
- UniProt
- P49773
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