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BID 抗体 (AA 1-195)

BID 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN3042321) (BID 抗体 (AA 1-195))

Anti-Bid Picoband antibody, IHC(P): Human Mammary Cancer Tissue (BID 抗体 (AA 1-195))

Anti-Bid Picoband antibody, ICC: A549 Cell (BID 抗体 (AA 1-195))

Anti-Bid Picoband antibody, IHC(P): Mouse Spleen Tissue (BID 抗体 (AA 1-195))

Anti-Bid Picoband antibody, IHC(P): Rat Intestine Tissue (BID 抗体 (AA 1-195))

5 images

(6 references)

产品编号 ABIN3042321

发货至: 中国

Datasheet as PDF

抗原 See all BID 抗体

BID (BH3 Interacting Domain Death Agonist (BID))
抗原表位
17

17

15

8

8

7

4

4

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-195
适用
119

63

28

1

1

1
人, 小鼠, 大鼠
宿主
129

16
兔
Compatible Secondaries
克隆类型
120

25
多克隆
标记
71

11

11

6

5

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2
This BID antibody is un-conjugated
应用范围
118

53

44

34

25

25

24

15

12

10

7

5

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

原理

Anti-BID Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-BID Antibody Picoband® (ABIN3042321). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.

亚型

IgG

Top Product

Discover our top product BID Primary Antibody
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
BID 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) antibody
BID 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3C5 unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 68-103) antibody
BID 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB0957-RB0958 unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-196) antibody
BID 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 68-103) antibody
BID 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
BID 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
BID 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F3-1A3 unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 39-68) antibody
BID 适用: 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) antibody
BID 适用: 人 WB, IHC (p), IF, IF (cc) 宿主: 小鼠 Monoclonal 9C7 unconjugated

anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
BID 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. 2. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. 3. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Wang, Gong, Chen, Xiong, Zhou, Huang, Kong: "NLRP3 inflammasome sequential changes in Staphylococcus aureus-induced mouse model of acute rhinosinusitis." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15806-20, (2015) (PubMed).

Li, Chen, Zhang, Song, Mu: "Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats." in: Neural regeneration research, Vol. 7, Issue 5, pp. 325-31, (2015) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Yao, Peng, Peng, Tan, Wu, Wu, Chen, Li, Li, Zhu: "Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye." in: International journal of ophthalmology, Vol. 3, Issue 2, pp. 114-9, (2012) (PubMed).

Wang, Liu, Mi, Wang, Tang, Zhao, Li: "Sonodynamically induced apoptosis by protoporphyrin IX on hepatoma-22 cells in vitro." in: Ultrasound in medicine & biology, Vol. 36, Issue 4, pp. 667-76, (2010) (PubMed).
抗原

BID (BH3 Interacting Domain Death Agonist (BID))

别名

BID (BID 产品)

背景

Synonyms: Antigen KI-67,MKI67,
Tissue Specificity: Released primarily from activated T lymphocytes.
Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Sequence Similarities: Contains 1 FHA domain.

分子量

22 kDa

基因ID

637

UniProt

P55957

途径

Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

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