The stated application concentrations are suggested starting points. Titration of the GLUT4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the GLUT4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
抗原
GLUT4 (SLC2A4)
(Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))
GLUT4 antibody, Glut-4 antibody, Glut4 antibody, glut4 antibody, slc2a4 antibody, SLC2A4 antibody, solute carrier family 2 member 4 antibody, solute carrier family 2 (facilitated glucose transporter), member 4 antibody, solute carrier family 2 (facilitated glucose transporter), member 4 L homeolog antibody, SLC2A4 antibody, Slc2a4 antibody, GLUT4 antibody, slc2a4.L antibody
背景
Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.