The stated application concentrations are suggested starting points. Titration of the SIRT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the SIRT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
NAD-dependent deacetylase sirtuin-3, mitochondrial is a protein that in humans is encoded by the SIRT3 gene. It is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression in cultured cells increases respiration and decreases the production of reactive oxygen species. It can function as a mitochondrial deacetylase. Additionally, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.