NLRP3 抗体 (N-Term)
Quick Overview for NLRP3 抗体 (N-Term) (ABIN3032021)
抗原
See all NLRP3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the N-terminus of human NLRP3 (RYLEDLEDVDLKKFKMHLED) was used as the immunogen for this NLRP3 antibody.
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亚型
- Ig Fraction
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anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-93), (Pyrin Domain) antibody
NLRP3 适用: 人, 小鼠 WB, IHC, IF, IP 宿主: 小鼠 Monoclonal Cryo-2 unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 15-120) antibodyNLRP3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, IHC (p), FACS, ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 671-1033) antibodyNLRP3 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (C-Term) antibodyVerified NLRP3 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (C-Term) antibodyNLRP3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 12-31), (N-Term) antibodyNLRP3 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 30-102) antibodyNLRP3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-100) antibodyNLRP3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 21-194) antibodyNLRP3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 65-91), (N-Term) antibodyNLRP3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB22404 unconjugated
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应用备注
- The stated application concentrations are suggested starting points. Titration of the NLRP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IF/ICC: 2-3 μg/mL,FACS: 1-3 μg/10^6 cells
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the NLRP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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别名
- NLRP3 (NALP3)
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背景
- NOD-like receptor family pyrin domain containing 3, also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. The gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the proteins nucleotide-binding domain reduced ATP binding, Caspase-1 activation, IL-1b production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for NLRP3 inflammasomes in antifungal immunity, Gross et al.showed that deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.
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UniProt
- Q96P20
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
抗原
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