HLA-DR 抗体
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北京 101111
Quick Overview for HLA-DR 抗体 (ABIN3027002)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Raji cells were used as the immunogen for the HLA-DR antibody.
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亚型
- IgG2a kappa
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anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, IHC, WB, ICC, IF (p) 宿主: 小鼠 Monoclonal LN3 unconjugated
anti-HLA-DR (HLA-DR) antibody (FITC)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 FITC
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, IHC, WB 宿主: 小鼠 Monoclonal TAL 1B5 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR 适用: 人, 犬, Non-Human Primate FACS 宿主: 小鼠 Monoclonal L243 PE
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-12 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 猴 FACS, IHC, WB, IF 宿主: 小鼠 Monoclonal SPM289 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func 宿主: 小鼠 Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PerCP)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 PerCP
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal HL-39 unconjugated
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应用备注
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Optimal dilution of the HLA-DR antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- HLA-DR
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别名
- HLA-DR (MHC II)
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背景
- This mAb reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
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途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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