HLA-DR 抗体
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北京 101111
Quick Overview for HLA-DR 抗体 (ABIN6939641)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- HLA-DRB (MHC II) Antibody
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特异性
- This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Activated human peripheral blood mononuclear cells
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亚型
- IgG2b, kappa
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anti-HLA-DR (HLA-DR) antibody
HLA-DR 适用: 人 FACS, IHC, WB, ICC, IF (p) 宿主: 小鼠 Monoclonal LN3 unconjugated
anti-HLA-DR (HLA-DR) antibody (FITC)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 FITC
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, IHC, WB 宿主: 小鼠 Monoclonal TAL 1B5 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR 适用: 人, 犬, Non-Human Primate FACS 宿主: 小鼠 Monoclonal L243 PE
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-12 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人, 犬, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func 宿主: 小鼠 Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PerCP)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 PerCP
anti-HLA-DR (HLA-DR) antibodyHLA-DR 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal HL-39 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-12 PE
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应用备注
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Positive Control: Raji, Ramos, Daudi or HuT78 cells. Spleen, Tonsil or lymph node.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- HLA-DR
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别名
- HLA-DR
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背景
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DR alpha chain, DRB1, DRB4, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DR1B, HLA DR3B, HLA DRA, HLA DRA1, HLA DRB1, HLA DRB3, HLA DRB4, HLA DRB5, HLA-DRA, HLADR4B, HLADRA1, HLADRB, Major histocompatibility complex class II DR alpha, Major histocompatibility complex class II DR beta 1, Major histocompatibility complex class II DR beta 3, Major histocompatibility complex class II DR beta 4, Major histocompatibility complex class II DR beta 5, MHC cell surface glycoprotein, MHC class II antigen DRA
Daudi or HuT78 cells. Spleen, Raji, Ramos, tonsil or lymph node.
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分子量
- ~28kDa (beta chain)
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基因ID
- 3123, 520048
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UniProt
- P01903
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途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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