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BCL2L1 抗体

This anti-BCL2L1 antibody is a 小鼠 单克隆 antibody detecting BCL2L1 in WB, IF, FACS 和 IHC (p). Suitable for 人, 小鼠, 大鼠 和 Pig.
产品编号 ABIN3026614
发货至: 中国

Quick Overview for BCL2L1 抗体 (ABIN3026614)

抗原

See all BCL2L1 抗体
BCL2L1 (BCL2-Like 1 (BCL2L1))

适用

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人, 小鼠, 大鼠, Pig

宿主

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小鼠

克隆类型

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单克隆

标记

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This BCL2L1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

BX006
  • 产品特性

    This antibody recognizes a protein of 27 kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

    纯化方法

    Protein G affinity chromatography

    免疫原

    Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).

    亚型

    IgG2a
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
    2. This antibody reacts with both BclXS and BclXL.
    3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (3)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    BCL2L1 (BCL2-Like 1 (BCL2L1))

    别名

    Bcl-X

    背景

    This antibody recognizes a protein of 27  kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

    基因ID

    598

    途径

    Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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