CD45 抗体
Quick Overview for CD45 抗体 (ABIN3026316)
抗原
See all CD45 (PTPRC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody cocktail contains an antibody which binds to all CD45 leukocyte common antigen (LCA) family isoforms (clone 2B11), and an antibody specific to the CD45RB isoform (clone PD7/26). CD45 is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of the CD45 isoforms have protein phosphatase activity and are involved in regulation of transmembrane signals. antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this antibody cocktail is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody cocktail detects CD45 expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Isolated neoplastic cells from T cell lymphoma were used as the immunogen for clone 2B11 and human peripheral blood lymphocytes maintained in Tcell growth factor were used as the immunogen for clone PD7/26.
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亚型
- IgG1 kappa
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anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC 适用: 人, 犬 FACS, IHC, WB, StM 宿主: 小鼠 Monoclonal 2B11-PD7-26 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 193-575) antibodyPTPRC 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 371-556) antibodyPTPRC 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 24-575) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 小鼠 Monoclonal 17H9D4 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (C-Term) antibodyPTPRC 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Cat, 兔, 绵羊, 豚鼠, Pig, 山羊, 马, 猴, Hamster, 驴, Avian WB, IHC (p), IHC (f) 宿主: 山羊 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, StM 宿主: 兔 Monoclonal PTPRC-2877R unconjugated Recombinant Antibody
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-28 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 431-577) antibodyPTPRC 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1B9B10 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 378-577) antibodyPTPRC 适用: 人, 小鼠, 大鼠 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3E2 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 928-989) antibodyPTPRC 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 5D3A3 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD 45 antibody cocktail to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD45 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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别名
- CD45
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背景
- This antibody cocktail contains an antibody which binds to all CD45 leukocyte common antigen (LCA) family isoforms (clone 2B11), and an antibody specific to the CD45RB isoform (clone PD7/26). CD45 is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of the CD45 isoforms have protein phosphatase activity and are involved in regulation of transmembrane signals. antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this antibody cocktail is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody cocktail detects CD45 expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
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基因ID
- 5788
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
抗原
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