小鼠 anti-人 IgG4 (Fc Region) Antibody
IF, FACS, IHC (p)
Monoclonal
IHCG4-1
IgG1
unconjugated
1 image
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- 抗原 See all IgG4 products
- IgG4
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A human recombinant protein corresponding to the Fc region was used as the immunogen for this IgG4 antibody.
- 克隆位点
- IHCG4-1
- 亚型
- IgG1
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IgG4 antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue is enhanced by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
- 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the IgG4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- IgG4
- Abstract
- IgG4 产品
- 物质类
- Antibody
- 背景
- The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.
- 基因ID
- 3503
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