Connexin 43/GJA1 抗体 (C-Term)
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Quick Overview for Connexin 43/GJA1 抗体 (C-Term) (ABIN302618)
抗原
See all Connexin 43/GJA1 (GJA1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody detects Connexin43 at C-terminal domain.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- GJA1 antibody was raised against synthetic peptide from C-terminal domain of human GJA1 / Connexin 43.
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应用备注
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Immunohistochemistry on paraffin sections (10 - 20 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % sodium azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.
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- Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))
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别名
- GJA1 / Cx43
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背景
- Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
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基因ID
- 2697
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NCBI登录号
- NP_000156
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UniProt
- P17302
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途径
- MAPK Pathway, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
抗原
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