S100A8/A9 Complex (Calprotectin) 抗体
S100A8/A9
适用: 人, Cow, Pig, Cat, 犬, 豚鼠, 马, 猴, 兔, 大鼠, Baboon, 山羊, 小鼠
FACS, IF, IHC (p)
宿主: 小鼠
Monoclonal
MAC387
unconjugated
1 image
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- 抗原 See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
- 适用
- 人, Cow, Pig, Cat, 犬, 豚鼠, 马, 猴, 兔, 大鼠, Baboon, 山羊, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- An affinity purified monocyte membrane preparation was used as the immunogen for the MRP8 + MPR14 antibody.
- 克隆位点
- MAC387
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product S100A8/A9 Primary Antibody
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- 应用备注
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Optimal dilution of the MRP8 + MPR14 antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
- 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the MRP8 + MPR14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
- 别名
- MRP8 + MPR14 (S100A8/A9 产品)
- 别名
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, S100 calcium binding protein A9 antibody, S100A9 antibody
- 背景
- Recognizes S100A8/MPR8 and S100A9/MRP14, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
- 途径
- S100 Proteins
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