ICAM-3/CD50 抗体
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- 抗原 See all ICAM-3/CD50 (ICAM3) 抗体
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ICAM-3/CD50 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant human protein was used as the immunogen for the ICAM3 antibody.
- 克隆位点
- ICAM3-1019
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Primary Antibody
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anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody
ICAM3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-171 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibodyICAM3 适用: 人 FACS, IHC (p), Func 宿主: 小鼠 Monoclonal MEM-04 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-203) antibodyICAM3 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 2H10G7 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 200-547) antibodyICAM3 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) antibodyICAM3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F8 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin)ICAM3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-171 Biotin
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (PE)ICAM3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-171 PE
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 46-197) antibodyICAM3 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-130) antibodyICAM3 适用: 人 ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 259-491) antibodyICAM3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Optimal dilution of the ICAM3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the ICAM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 别名
- ICAM3 (ICAM3 产品)
- 别名
- CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
- 背景
- Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.
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