CD86 抗体
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- 抗原 See all CD86 抗体
- CD86
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD86 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
- 克隆位点
- C86-1146
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD86 Primary Antibody
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anti-CD86 (CD86) (AA 140-175) antibody
CD86 适用: 人, 小鼠, 大鼠, 犬 FACS, WB, ELISA, IHC (fro), ICC, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD86 (CD86) (AA 37-272) antibodyCD86 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD86 (CD86) antibodyCD86 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, ELISA, IF, BP, StM, Coat 宿主: 兔 Monoclonal C86-2160R unconjugated Recombinant Antibody
anti-CD86 (CD86) antibodyCD86 适用: 人, 小鼠, 大鼠 FACS, IHC, IF, Func, BP, StM 宿主: 小鼠 Monoclonal BU63 unconjugated
anti-CD86 (CD86) antibodyCD86 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal BU63 unconjugated
anti-CD86 (CD86) antibody (FITC)CD86 适用: 人 FACS 宿主: 小鼠 Monoclonal BU63 FITC
anti-CD86 (CD86) antibodyCD86 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-CD86 (CD86) (AA 30-247) antibodyCD86 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD86 (CD86) antibodyCD86 适用: 人, 小鼠, 大鼠 FACS, IHC, IF, BP, StM 宿主: 小鼠 Monoclonal rC86-1146 unconjugated Recombinant Antibody
anti-CD86 (CD86) antibodyCD86 适用: 人, 小鼠, 大鼠 WB, IHC, IF, BP, StM 宿主: 小鼠 Monoclonal C86-1146 unconjugated
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- 应用备注
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Optimal dilution of the CD86 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)
- 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- CD86
- 别名
- CD86 (CD86 产品)
- 别名
- B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
- 背景
- Recognizes a protein of 70 kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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